Anti-Thymidine Kinase 1 antibody [3B3.E11] (ab988)

Overview

  • Product nameAnti-Thymidine Kinase 1 antibody [3B3.E11]
    See all Thymidine Kinase 1 primary antibodies
  • Description
    Mouse monoclonal [3B3.E11] to Thymidine Kinase 1
  • SpecificityThis antibody recognizes Thymidine Kinase 1. Only very weak cross reactivity with other TKs has been observed.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Amino terminal-His-tagged recombinant human thymidine kinase.

  • Positive control
    • H630R10 colon cancer cell lysate or HT1080 cell lysate IF/ICC: HeLa cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS, pH 7.0
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number3B3.E11
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use a concentration of 2 - 5 µg/ml. (optimized for TK on solid phase at 10 µg/ml). Dilute in PBS or medium which is identical to that used in the assay system.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 30 kDa.

Target

  • Sequence similaritiesBelongs to the thymidine kinase family.
  • Post-translational
    modifications
    Phosphorylated on Ser-13 in mitosis.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Anti-Thymidine Kinase 1 antibody [3B3.E11] images

  • ICC/IF image of ab988 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab988, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Thymidine Kinase 1 antibody [3B3.E11] (ab988) at 1/500 dilution + Whole cell lysate prepared from human SW620 cells at 25 µg

    Secondary
    HRP conjugated pig dakocytomation at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab988 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab988, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Thymidine Kinase 1 antibody [3B3.E11] (ab988)

This product has been referenced in:
  • Chan F  et al. Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activity. Mol Cancer Ther 6:3147-57 (2007). Read more (PubMed: 18089709) »
  • Tu Z  et al. IKK{alpha} Regulates Estrogen-induced Cell Cycle Progression by Modulating E2F1 Expression. J Biol Chem 281:6699-706 (2006). Read more (PubMed: 16407216) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblast)
Loading amount 10000 cells
Specification Human fibroblast
Treatment Rb inactivation by viral kinase
Gel Running Conditions Reduced Denaturing (4-20% acrylamide SDS-PAGE)
Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Sep 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW620)
Loading amount 25 µg
Specification SW620
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HELA cell)
Loading amount 40 µg
Specification HELA cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2007

I'm sorry to hear you are having problem with ab988 lot 50818. This lot is actually originating from the same batch as lot 62847 which your customer has reported was working and therefore we must conclude there is a problem with the vial you receiv...

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Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. This is one of our most frequently-ordered antibodies in the area of cancer research. The customers we have spoken with recently have been very pleas...

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To the best of our knowledge, there are no publications featuring this antibody. This antibody is expected to recognise both the native and denatured form of TK. The neutralisation potential of this antibody has not been determined.

The exact epitope detected by this antibody has not been determined.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"