Overview

  • Product name
    Anti-Thymidine Kinase 1 antibody
    See all Thymidine Kinase 1 primary antibodies
  • Description
    Rabbit polyclonal to Thymidine Kinase 1
  • Specificity
    ab59271 detects endogenous levels of total Thymidine Kinase protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized non-phosphopeptide derived from human Thymidine Kinase around the phosphorylation site of serine 13.

  • Positive control
    • Extracts from COLO205 cells; human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab59271 at a dilution of 1/50. Left hand image - without immunising peptide; right hand image - with immunising peptide.
  • All lanes : Anti-Thymidine Kinase 1 antibody (ab59271) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells with no immunising peptide
    Lane 2 : extracts from COLO205 cells with immunising peptide


    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References

This product has been referenced in:
  • Wei Q  et al. Anticancer activity of a thymidine quinoxaline conjugate is modulated by cytosolic thymidine pathways. BMC Cancer 15:159 (2015). IHC . Read more (PubMed: 25881156) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-11%)
Sample
Rat Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2013

Thank you for your enquiry.

I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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Thank you for contacting us.

To our knowledge this product has not been tested for cross reactivity against Thymidine kinase 2 (TK2; SwissProt ID O00142). However, as the homology of the two proteins is extremely poor at only 14%, we do not f...

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Glad to hear that the replacement vial was successfull. I checked our stocks and we have 3 more vials of ab59271, lot GR54001-1 in stock. It is the only lot in stock right now but just in case, simply request the specific lot you are interested in purc...

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Thanks for  your reply. So you would like 1 vial of ab59271 of a different lot and 2 vials of ab47681. That leaves you with 1 more antibody to replace the 4 vials of ab59271 you tested that did not work. Please let me know what add...

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Thank you for sending along the purchase order numbers. I see they are all for purchase of ab59271, lot number 578728. Have you tried each vial of antibody to determine if perhaps it is a vial-specific issue?

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HELA Cell)
Loading amount
40 µg
Specification
HELA Cell
Gel Running Conditions
Non-reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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