Overview

  • Product nameAnti-Thymidine Kinase 1 antibody
    See all Thymidine Kinase 1 primary antibodies
  • Description
    Rabbit polyclonal to Thymidine Kinase 1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab75277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similaritiesBelongs to the thymidine kinase family.
  • Post-translational
    modifications
    Phosphorylated on Ser-13 in mitosis.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Anti-Thymidine Kinase 1 antibody images

  • All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 130 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
    ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-Thymidine Kinase 1 antibody (ab75277)

ab75277 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75277.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"