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Rabbit polyclonal to Thymidine Kinase 1
Predicted to work with:
Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to a region between residues 205 and 234 of human Thymidine Kinase (NP_003249.1)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab75277 was affinity purified using an epitope specific to Thymidine Kinase immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use at 2-5 µg/mg of lysate.
Belongs to the thymidine kinase family.
Phosphorylated on Ser-13 in mitosis.
Information by UniProt
Thymidine kinase 1 antibody
Western blot - Anti-Thymidine Kinase 1 antibody (ab75277)
All lanes : Anti-Thymidine Kinase 1 antibody (ab75277) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 25 kDa Observed band size: 25 kDa Additional bands at: 130 kDa, 65 kDa. We are unsure as to the identity of these extra bands. Exposure time: 30 seconds
Immunoprecipitation - Anti-Thymidine Kinase 1 antibody (ab75277)
Detection of Thymidine Kinase by Western Blot of Immunprecipitate.
ab75277 at 1µg/ml staining Thymidine Kinase in HeLa whole cell lysate immunoprecipitated using ab75277 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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