Validated using a knockout cell line

Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495)


  • Product nameAnti-Thymidine Kinase 1 antibody [EPR3193]
    See all Thymidine Kinase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3193] to Thymidine Kinase 1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Thymidine Kinase 1 aa 1-100. The exact sequence is proprietary.

  • Positive control
    • WB: Molt-4 cell lysate; IHC-P: human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Thymidine Kinase 1 antibody (Alexa Fluor® 488) [EPR3193] (ab201259)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3193
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab76495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/5000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IP 1/10.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Sequence similaritiesBelongs to the thymidine kinase family.
  • Post-translational
    Phosphorylated on Ser-13 in mitosis.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytosolic antibody
    • KITH_HUMAN antibody
    • Thymidine kinase 1 antibody
    • Thymidine kinase 1 soluble antibody
    • Thymidine kinase 1 soluble isoform antibody
    • Thymidine kinase antibody
    • Thymidine kinase cytosolic antibody
    • TK 1 antibody
    • TK 2 antibody
    • TK1 antibody
    • Tk1a antibody
    • Tk1b antibody
    • TK2 antibody
    see all

Anti-Thymidine Kinase 1 antibody [EPR3193] images

  • Predicted band size : 25 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: MOLT4 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76495 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76495 was shown to specifically react with TK1 when TK1 knockout samples were used. Wild-type and TK1 knockout samples were subjected to SDS-PAGE.  Ab76495 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 ab76495
  • Overlay histogram showing HeLa cells stained with ab76495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76495, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495) at 1/50000 dilution + Molt-4 cell lysate at 10 µg

    HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

References for Anti-Thymidine Kinase 1 antibody [EPR3193] (ab76495)

This product has been referenced in:
  • Breitbach CJ  et al. Oncolytic vaccinia virus disrupts tumor-associated vasculature in humans. Cancer Res 73:1265-75 (2013). Read more (PubMed: 23393196) »
  • Viel T  et al. Early assessment of the efficacy of temozolomide chemotherapy in experimental glioblastoma using [18F]FLT-PET imaging. PLoS One 8:e67911 (2013). IHC-P ; Mouse . Read more (PubMed: 23861829) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

I'm sorry to confirm this antibody is not tested in IHC on paraffin sections (IHC-P). All tested species and applications covered by the 6 month guarantee are listed on the individual datasheets. These are updated...

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Thank you for contacting Abcam.

The main difference between ab76495 and ab91651 is that they will have different epitope recognition sites.

Also for ab76495, we guarantee it to react with mouse and human tissue in the following appl...

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Thank you very much for your enquiry. Based on the immunogen sequences both Thymidine Kinase 1 (TK1) antibodies ab76495 and ab91651 are specific for TK1 and will not recognise TK2: The immunogen sequence for ab76495 is a peptide near the N-termin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (T cells)
Specification T cells
Fixation Formaldehyde
Permeabilization Yes - SAPONIN
Gating Strategy CD4 T cells, live

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Submitted Oct 30 2009