The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
FunctionMay have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
PathwayPyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2.
Involvement in diseaseMitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Western blot - Anti-Thymidine Phosphorylase antibody (ab69120)
All lanes : Anti-Thymidine Phosphorylase antibody (ab69120) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa Whole Cell Lysate - Bleomycin Treated (40 µM) Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa Observed band size : 50 kDa Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Thymidine Phosphorylase antibody (ab69120)
This product has been referenced in:
Torres-Torronteras J et al. Gene Therapy Using a Liver-targeted AAV Vector Restores Nucleoside and Nucleotide Homeostasis in a Murine Model of MNGIE. Mol TherN/A:N/A (2014).
Read more (PubMed: 24448160) »