Anti-Thymidylate Synthase antibody (ab34836)

Overview

  • Product nameAnti-Thymidylate Synthase antibody
    See all Thymidylate Synthase primary antibodies
  • Description
    Rabbit polyclonal to Thymidylate Synthase
  • SpecificityThis antibody recognizes Thymidylate Synthase and an additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. (This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.) The antibody can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
  • Tested applicationsSuitable for: ELISA, IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, African Green Monkey
  • Immunogen

    Recombinant full length protein (Human) expressed in E. coli.

  • Positive control
    • HeLa cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab34836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
IP 1/500.
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

Anti-Thymidylate Synthase antibody images

  • ICC/IF image of ab34836 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Thymidylate Synthase antibody (ab34836)

ab34836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"