Overview

  • Product name
    Anti-Thymidylate Synthase antibody
    See all Thymidylate Synthase primary antibodies
  • Description
    Mouse monoclonal to Thymidylate Synthase
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC , corresponding to amino acids 111-211 of Human Thymidylate Synthase

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab108995 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab58287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration. PubMed: 23840376

Target

  • Function
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • Pathway
    Pyrimidine metabolism; dTTP biosynthesis.
  • Sequence similarities
    Belongs to the thymidylate synthase family.
  • Cellular localization
    Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • d TMP synthase antibody
    • EC 2.1.1.45 antibody
    • HsT422 antibody
    • MGC88736 antibody
    • OTTHUMP00000162195 antibody
    • Thymidylate synthase antibody
    • Thymidylate synthetase antibody
    • TMS antibody
    • TS antibody
    • TSase antibody
    • Tyms antibody
    • TYMS protein antibody
    • Tyms thymidylate synthetase antibody
    • TYSY_HUMAN antibody
    see all

Images

  • Thymidylate Synthase antibody (ab58287) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 36 kDa
    Thymidylate Synthase antibody (ab58287) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab58287 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58287, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1 µg/ml

    Lane 1 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.1 µg
    Lane 2 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Overlay histogram showing HeLa cells stained with ab58287 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Wu R  et al. Histone H3K27 Trimethylation Modulates 5-Fluorouracil Resistance by Inhibiting PU.1 Binding to the DPYD Promoter. Cancer Res 76:6362-6373 (2016). WB . Read more (PubMed: 27578004) »
  • Del Bufalo D  et al. Histone deacetylase inhibition synergistically enhances pemetrexed cytotoxicity through induction of apoptosis and autophagy in non-small cell lung cancer. Mol Cancer 13:230 (2014). WB ; Human . Read more (PubMed: 25301686) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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