Overview

  • Product nameAnti-Thymidylate Synthase antibody
    See all Thymidylate Synthase primary antibodies
  • Description
    Mouse monoclonal to Thymidylate Synthase
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC , corresponding to amino acids 111-211 of Human Thymidylate Synthase

Properties

Applications

Our Abpromise guarantee covers the use of ab58287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration. PubMed: 23840376

Target

  • FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • PathwayPyrimidine metabolism; dTTP biosynthesis.
  • Sequence similaritiesBelongs to the thymidylate synthase family.
  • Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • d TMP synthase antibody
    • EC 2.1.1.45 antibody
    • HsT422 antibody
    • MGC88736 antibody
    • OTTHUMP00000162195 antibody
    • Thymidylate synthase antibody
    • Thymidylate synthetase antibody
    • TMS antibody
    • TS antibody
    • TSase antibody
    • Tyms antibody
    • TYMS protein antibody
    • Tyms thymidylate synthetase antibody
    • TYSY_HUMAN antibody
    see all

Anti-Thymidylate Synthase antibody images

  • Thymidylate Synthase antibody (ab58287) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 36 kDa
    Thymidylate Synthase antibody (ab58287) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab58287 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58287, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1 µg/ml

    Lane 1 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.1 µg
    Lane 2 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Overlay histogram showing HeLa cells stained with ab58287 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Thymidylate Synthase antibody (ab58287)

This product has been referenced in:
  • Del Bufalo D  et al. Histone deacetylase inhibition synergistically enhances pemetrexed cytotoxicity through induction of apoptosis and autophagy in non-small cell lung cancer. Mol Cancer 13:230 (2014). WB ; Human . Read more (PubMed: 25301686) »
  • Szulkin A  et al. Variation in drug sensitivity of malignant mesothelioma cell lines with substantial effects of selenite and bortezomib, highlights need for individualized therapy. PLoS One 8:e65903 (2013). ICC ; Human . Read more (PubMed: 23840376) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"