Anti-Thymidylate Synthase antibody (ab7398)
Key features and details
- Sheep polyclonal to Thymidylate Synthase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Thymidylate Synthase antibody
See all Thymidylate Synthase primary antibodies -
Description
Sheep polyclonal to Thymidylate Synthase -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Human Thymidylate Synthase. Produced in E.coli.
Database link: P04818 -
General notes
The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
Pathway
Pyrimidine metabolism; dTTP biosynthesis. -
Sequence similarities
Belongs to the thymidylate synthase family. -
Cellular localization
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7298 Human
- Omim: 188350 Human
- SwissProt: P04818 Human
- Unigene: 369762 Human
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Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Images
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ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.
Datasheets and documents
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Datasheet download
References (7)
ab7398 has been referenced in 7 publications.
- Zhang L et al. Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN. EBioMedicine 43:473-486 (2019). PubMed: 31060905
- Gu YJ et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . PubMed: 26378040
- MacFarlane AJ et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). PubMed: 24500934
- Macfarlane AJ et al. Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA. J Biol Chem 286:44015-22 (2011). WB ; Mouse . PubMed: 22057276
- Gay S et al. Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions. EMBO Rep 11:698-704 (2010). WB ; Chinese hamster . PubMed: 20671737
- Fox JT et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem 284:31097-108 (2009). WB, IP ; Human . PubMed: 19734144
- Woeller CF et al. Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway. J Biol Chem 282:17623-31 (2007). PubMed: 17446168