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Product name Anti-Thymidylate Synthase antibody [EPR4545] See all Thymidylate Synthase primary antibodies
Rabbit monoclonal [EPR4545] to Thymidylate Synthase
Tested applications Suitable for:
WB, IP, IHC-P, ICC, Flow Cyt more details
Mouse, Rat, Human
Synthetic peptide, corresponding to residues from the C-terminus of Human Thymidylate Synthase.
HeLa, Jurkat, HL60 and MCF7 cell lysates; Human ovary carcinoma tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Thymidylate Synthase antibody (Alexa Fluor[EPR4545] ( ® 488) ab197883) Anti-Thymidylate Synthase antibody (Alexa Fluor[EPR4545] ( ® 647) ab197899) Anti-Thymidylate Synthase antibody (HRP) [EPR4545] ( ab198599)
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR4545
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
1/10 - 1/100.
1/50 - 1/100. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
1/100 - 1/250.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Function Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Pathway Pyrimidine metabolism; dTTP biosynthesis.
Sequence similarities Belongs to the thymidylate synthase family.
Cellular localization Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
Information by UniProt
d TMP synthase antibody
EC 220.127.116.11 antibody
Anti-Thymidylate Synthase antibody [EPR4545] images
Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HL60 cell lysate Lane 4 : MCF7 cell lysate Lysates/proteins at 10 µg per lane. Performed under reducing conditions. Predicted band size : 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.
References for Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
This product has been referenced in:
et al. Dasatinib modulates sensitivity to pemetrexed in malignant pleural mesothelioma cell lines. Oncotarget 7:76577-76589 (2016).
Read more (PubMed: 27391433) »
et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015).
Read more (PubMed: 25860483) »
See all 5 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"