Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab108995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-Thymidylate Synthase antibody [EPR4545] images

  • All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 36 kDa
  • ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.

References for Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

This product has been referenced in:
  • Lund K  et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015). WB . Read more (PubMed: 25860483) »
  • Allen MD  et al. Prognostic and therapeutic impact of argininosuccinate synthetase 1 control in bladder cancer as monitored longitudinally by PET imaging. Cancer Res 74:896-907 (2014). Read more (PubMed: 24285724) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"