Overview

  • Product nameAnti-Thymine DNA glycosylase antibody
    See all Thymine DNA glycosylase primary antibodies
  • Description
    Rabbit polyclonal to Thymine DNA glycosylase
  • Specificityab65219 detects endogenous levels of total Thymine DNA glycosylase protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Thymine DNA glycosylase

  • Positive control
    • Jurkat cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65219 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionIn the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • Sequence similaritiesBelongs to the TDG/mug DNA glycosylase family.
  • Post-translational
    modifications
    Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C JUN leucine zipper interactive protein antibody
    • C JUN leucine zipper interactive protein antibody
    • C-JUN leucine zipper interactive protein JZA-3 antibody
    • E130317C12Rik antibody
    • EC 3.2.2.29 antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch-specific thymine DNA glycosylase antibody
    • JZA 3 antibody
    • Jza1 antibody
    • T:G mismatch thymine glycosylase antibody
    • Tdg antibody
    • TDG_HUMAN antibody
    • Thymine DNA glycosylase antibody
    • Thymine-DNA glycosylase antibody
    see all

Anti-Thymine DNA glycosylase antibody images

  • All lanes : Anti-Thymine DNA glycosylase antibody (ab65219) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Thymine DNA glycosylase antibody (ab65219)

This product has been referenced in:
  • Nettersheim D  et al. Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development. PLoS One 8:e82881 (2013). WB ; Human . Read more (PubMed: 24386123) »
  • Swarnalatha M  et al. The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle. Nucleic Acids Res : (2012). Read more (PubMed: 22772991) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam. The immunogen sequence for ab65219 is from amino acids ****** of human TDG. If there is anything else I can help with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"