Recombinant
RabMAb

Anti-Thymine DNA glycosylase antibody [EPR8774] (ab154192)

Overview

  • Product name
    Anti-Thymine DNA glycosylase antibody [EPR8774]
    See all Thymine DNA glycosylase primary antibodies
  • Description
    Rabbit Monoclonal [EPR8774] to Thymine DNA glycosylase
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Thymine DNA glycosylase (Q13569)

  • Positive control
    • Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
    • Sequence similarities
      Belongs to the TDG/mug DNA glycosylase family.
    • Post-translational
      modifications
      Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C JUN leucine zipper interactive protein antibody
      • C JUN leucine zipper interactive protein antibody
      • C-JUN leucine zipper interactive protein JZA-3 antibody
      • E130317C12Rik antibody
      • EC 3.2.2.29 antibody
      • G/T mismatch specific thymine DNA glycosylase antibody
      • G/T mismatch specific thymine DNA glycosylase antibody
      • G/T mismatch-specific thymine DNA glycosylase antibody
      • JZA 3 antibody
      • Jza1 antibody
      • T:G mismatch thymine glycosylase antibody
      • Tdg antibody
      • TDG_HUMAN antibody
      • Thymine DNA glycosylase antibody
      • Thymine-DNA glycosylase antibody
      see all

    Images

    • All lanes : Anti-Thymine DNA glycosylase antibody [EPR8774] (ab154192) at 1/5000 dilution

      Lane 1 : Mouse brain tissue at 20 µg
      Lane 2 : mouse heart tissue at 20 µg
      Lane 3 : mouse kidney tissue
      Lane 4 : mouse spleen tissue at 20 µg
      Lane 5 : rat brain tissue at 20 µg
      Lane 6 : rat heart tissue at 20 µg
      Lane 7 : rat kidney tissue at 20 µg
      Lane 8 : rat spleen tissue at 20 µg


      Predicted band size : 46 kDa
      Observed band size : 55-60 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • All lanes : Anti-Thymine DNA glycosylase antibody [EPR8774] (ab154192) at 1/1000 dilution

      Lane 1 : Fetal lung cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : Fetal brain cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 46 kDa
    • Flow cytometric analysis of permeabilized HeLa cells labeling Thymine DNA glycosylase with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green).

    References

    This product has been referenced in:
    • Neri F  et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics. Cell Rep N/A:N/A (2015). Read more (PubMed: 25660018) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (primary human fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Glycine)
    Loading amount
    40 µg
    Treatment
    control or anti-TDG siRNA
    Specification
    primary human fibroblasts
    Blocking step
    Odyssey-Blocking buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Enni Markkanen

    Verified customer

    Submitted Feb 09 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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