Overview

  • Product nameAnti-Thymocytes antibody
  • Description
    Rabbit polyclonal to Thymocytes
  • Specificityab112216 is strongly cytotoxic to Mouse thymocytes of all strains tested. ab112216 dilution which results in 50% cytotoxicity against thymus cells: 1/40000. Treatment of C57BL/6 splenocytes with ab112216 plus complement resulted in a reduction in the number of plaque-forming cells. As assessed by a CTL assay, T cell function was essentially eliminated in both pre-sensitized and post-sensitized treated samples. These results are consistent with the removal of T helper and T cytotoxicity cell activity.
  • Tested applicationsSuitable for: Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse Thymocytes

  • Positive control
    • Mouse Thymus (Tested strains: C3H/He, AKR, A.TH, A.TL, BALB/c); C57BL/6 Splenocytes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferConstituent: 100% Whole serum
  • Concentration information loading...
  • PurityPreadsorbed antiserum
  • Purification notesHeat Inactivation: 60 minutes at 56°C Adsorption: Mouse erythrocytes/hepatocytes
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies 1/20 - 1/5000.
Use at 1/1000 for depleting a cell population of Mouse thymocytes.

Target

  • RelevanceThymocytes are T cell precursors which develop in the thymus. The processes of beta-selection, positive selection and negative selection shape thymocytes into a peripheral pool of T cells that are able to respond to foreign pathogens and are immunologically tolerant towards self antigens.
  • Cellular localizationCell Membrane

Anti-Thymocytes antibody images

  • Antiserum Titration determined by a two stage cytotoxicity assay:

    Cell Source: Thymus
    Donors: BALB/c
    Cell Concentration: 1.1x106 cells per ml

References for Anti-Thymocytes antibody (ab112216)

ab112216 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab112216.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"