Overview

  • Product name
    Anti-Thymocytes antibody
  • Description
    Rabbit polyclonal to Thymocytes
  • Specificity
    ab112216 is strongly cytotoxic to Mouse thymocytes of all strains tested. ab112216 dilution which results in 50% cytotoxicity against thymus cells: 1/40000. Treatment of C57BL/6 splenocytes with ab112216 plus complement resulted in a reduction in the number of plaque-forming cells. As assessed by a CTL assay, T cell function was essentially eliminated in both pre-sensitized and post-sensitized treated samples. These results are consistent with the removal of T helper and T cytotoxicity cell activity.
  • Tested applications
    Suitable for: Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse Thymocytes

  • Positive control
    • Mouse Thymus (Tested strains: C3H/He, AKR, A.TH, A.TL, BALB/c); C57BL/6 Splenocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituent: 100% Whole serum
  • Concentration information loading...
  • Purity
    Preadsorbed antiserum
  • Purification notes
    Heat Inactivation: 60 minutes at 56°C Adsorption: Mouse erythrocytes/hepatocytes
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies 1/20 - 1/5000.
Use at 1/1000 for depleting a cell population of Mouse thymocytes.

Target

  • Relevance
    Thymocytes are T cell precursors which develop in the thymus. The processes of beta-selection, positive selection and negative selection shape thymocytes into a peripheral pool of T cells that are able to respond to foreign pathogens and are immunologically tolerant towards self antigens.
  • Cellular localization
    Cell Membrane

Images

  • Antiserum Titration determined by a two stage cytotoxicity assay:

    Cell Source: Thymus
    Donors: BALB/c
    Cell Concentration: 1.1x106 cells per ml

References

ab112216 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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