Anti-Thymosin alpha 1 antibody (ab76557)

Overview

  • Product nameAnti-Thymosin alpha 1 antibody
    See all Thymosin alpha 1 primary antibodies
  • Description
    Mouse monoclonal to Thymosin alpha 1
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-110 of Human Thymosin alpha 1

Properties

Applications

Our Abpromise guarantee covers the use of ab76557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Detects a band of approximately 38.1 kDa (predicted molecular weight: 12 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent concentration.

Target

  • FunctionProthymosin alpha may mediate immune function by conferring resistance to certain opportunistic infections.
  • Sequence similaritiesBelongs to the pro/parathymosin family.
  • Post-translational
    modifications
    Covalently linked to a small RNA of about 20 nucleotides.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Prothymosin alpha (gene sequence 28) antibody
    • Prothymosin alpha antibody
    • Prothymosin alpha like 1 protein antibody
    • PTMA antibody
    • PTMA_HUMAN antibody
    • Thymalfasin antibody
    • Thymosin alpha 1 acetate antibody
    • Thymosin alpha 1 antibody
    • Thymosin alpha antibody
    • Thymosin alpha-1 antibody
    • TMSA antibody
    see all

Anti-Thymosin alpha 1 antibody images

  • ab76557 staining Thymosin alpha 1 in human paraffin-embedded A549 lung cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed using the HOPE technique and permeabilized with 0.05% Tween. Samples were incubated with primary antibody (1/200) for 45 minutes at 25°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-Thymosin alpha 1 antibody (ab76557) at 5 µg/ml + immunizing immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

    Predicted band size : 12 kDa
    Observed band size : 38.1 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HL60 cells stained with ab76557 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76557, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Thymosin alpha 1 antibody (ab76557)

ab76557 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Zytomed Systems HRP Polymer Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Lung tissue)
Specification Lung tissue
Permeabilization No
Fixative HOPE-Fixation
Username

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Verified customer

Submitted Mar 12 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Paraffin-embedded A549 lung cancer cells)
Specification Paraffin-embedded A549 lung cancer cells
Permeabilization Yes - 0.05% TWEEN in wash buffer
Fixative HOPE
Username

Abcam user community

Verified customer

Submitted Apr 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"