Overview

  • Product nameAnti-Thymosin beta 10 antibody
    See all Thymosin beta 10 primary antibodies
  • Description
    Rabbit polyclonal to Thymosin beta 10
  • SpecificityHuman Thymosin B10 (aa 38-43). No cross reactivities with human Thymosin B4, Thymosin B9, Thymosin B15, Thymosin B4 (aa 1-4) peptide, or Thymosin B4 (aa 1-14).
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    KRSEIS

    conjugated to KLH, corresponding to amino acids 38-43 of Human Thymosin beta 10.

Properties

Applications

Our Abpromise guarantee covers the use of ab14338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4.
WB Use at an assay dependent concentration. Predicted molecular weight: 5 kDa. PubMed: 18854659
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-Thymosin beta 10 antibody images

  • ICC/IF image of ab14338 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14338, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14338 at 1/1000 dilution.

References for Anti-Thymosin beta 10 antibody (ab14338)

This product has been referenced in:
  • Lagarrigue M  et al. Revisiting Rat Spermatogenesis with MALDI Imaging at 20-{micro}m Resolution. Mol Cell Proteomics 10:M110.005991 (2011). IHC-P ; Rat . Read more (PubMed: 21149303) »
  • Zhang T  et al. Overexpression of thymosin beta-10 inhibits VEGF mRNA expression, autocrine VEGF protein production, and tube formation in hypoxia-induced monkey choroid-retinal endothelial cells. Ophthalmic Res 41:36-43 (2009). WB ; Human . Read more (PubMed: 18854659) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast)
Specification breast
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

Abcam user community

Verified customer

Submitted Jan 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"