• Product nameAnti-Thymosin beta 4 antibody
    See all Thymosin beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Thymosin beta 4
  • SpecificityHuman Thymosin B4 (aa 1-11)
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human Thymosin beta 4 aa 1-11 conjugated to keyhole limpet haemocyanin.


    Database link: P62328

  • Positive control
    • ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab14335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF 1/200.
IHC-P 1/100 - 1/1000.

Tested in lung and heart tissues.


  • FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
    Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase.
  • Tissue specificityExpressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
  • Sequence similaritiesBelongs to the thymosin beta family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fx antibody
    • Hematopoietic system regulatory peptide antibody
    • Prothymosin beta 4 antibody
    • PTMB 4 antibody
    • PTMB4 antibody
    • Seraspenide antibody
    • T beta 4 antibody
    • T beta-4 antibody
    • TB4X antibody
    • THYB 4 antibody
    • Thyb4 antibody
    • Thymosin beta 4 antibody
    • Thymosin beta 4 X chromosome antibody
    • Thymosin beta 4 X linked antibody
    • TMSB 4 antibody
    • TMSB4 antibody
    • Tmsb4x antibody
    • TYB4_HUMAN antibody
    see all

Anti-Thymosin beta 4 antibody images

  • ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.  

  • Indirect ELISA analysis. Anti-Thymosin beta 4 antibody (ab14335) at 1/1000 dilution. Sample: chicken heart culture serum. An alkaline phosphatase conjugated donkey anti-rabbit secondary antibody was used at 1/8000

    See Abreview

References for Anti-Thymosin beta 4 antibody (ab14335)

This product has been referenced in:
  • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »
  • Fu X  et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . Read more (PubMed: 25695679) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Xenopus laevis Cell (Brain)
Specification Brain
Permeabilization Yes - 0.01% Triton X-100
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Feb 27 2014

Application ELISA
Sample Chicken Serum (heart culture)
Specification heart culture
Type Indirect
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Yanyang Li

Verified customer

Submitted Jan 13 2014

Expiration date: 10th May 2013
Value: £235

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for ab101672:
Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
Positive control: hepatocarcinoma .

for ab14335:
No antigen retrieval is necessary, the protocol is as follows: Read More

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For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab1215...

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