Overview

  • Product name
    Anti-Thymosin beta 4 antibody
    See all Thymosin beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Thymosin beta 4
  • Specificity
    Human Thymosin B4 (aa 1-11)
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human Thymosin beta 4 aa 1-11 conjugated to keyhole limpet haemocyanin.
    Sequence:

    SDKPDMAEIEK


    Database link: P62328

  • Positive control
    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab14335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF 1/200.
IHC-P 1/100 - 1/1000.

Tested in lung and heart tissues.

Target

  • Function
    Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
    Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase.
  • Tissue specificity
    Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
  • Sequence similarities
    Belongs to the thymosin beta family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fx antibody
    • Hematopoietic system regulatory peptide antibody
    • Prothymosin beta 4 antibody
    • PTMB 4 antibody
    • PTMB4 antibody
    • Seraspenide antibody
    • T beta 4 antibody
    • T beta-4 antibody
    • TB4X antibody
    • THYB 4 antibody
    • Thyb4 antibody
    • Thymosin beta 4 antibody
    • Thymosin beta 4 X chromosome antibody
    • Thymosin beta 4 X linked antibody
    • TMSB 4 antibody
    • TMSB4 antibody
    • Tmsb4x antibody
    • TYB4_HUMAN antibody
    see all

Images

  • ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.  

  • Indirect ELISA analysis. Anti-Thymosin beta 4 antibody (ab14335) at 1/1000 dilution. Sample: chicken heart culture serum. An alkaline phosphatase conjugated donkey anti-rabbit secondary antibody was used at 1/8000

    See Abreview

References

This product has been referenced in:
  • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »
  • Fu X  et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . Read more (PubMed: 25695679) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Xenopus laevis Cell (Brain)
Specification
Brain
Permeabilization
Yes - 0.01% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 27 2014

Application
ELISA
Sample
Chicken Serum (heart culture)
Specification
heart culture
Type
Indirect
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Yanyang Li

Verified customer

Submitted Jan 13 2014

DISCOUNT CODE: xxxxxxx
Expiration date: 10th May 2013
Value: £235

I am pleased to hear you would like to participate in the AbTrial scheme. As discussed, I have generated the discount code above. This code will give you £23...

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for ab101672:
Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
Positive control: hepatocarcinoma .


for ab14335:
No antigen retrieval is necessary, the protocol is as follows: Read More

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab1215...

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