The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceThe THYN1 gene encodes a protein which is highly conserved among vertebrates and plant species, and may be involved in the induction of apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described.