Overview

  • Product nameAnti-Thyroglobulin antibody [EPR3614]
    See all Thyroglobulin primary antibodies
  • Description
    Rabbit monoclonal [EPR3614] to Thyroglobulin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human Thyroglobulin.

  • Positive control
    • WB: Human thyroid lysate IHC-P: Human papillary thyroid carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Predicted molecular weight: 305 kDa.
IP 1/50.
IHC-P 1/250 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22517745

Target

  • FunctionPrecursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
  • Tissue specificityThyroid gland specific.
  • Involvement in diseaseDefects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
    Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
  • Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
    Contains 11 thyroglobulin type-1 domains.
  • Post-translational
    modifications
    Sulfated tyrosines are desulfated during iodination.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AITD 3 antibody
    • AITD3 antibody
    • hTG antibody
    • TDH3 antibody
    • Tg antibody
    • TGN antibody
    • THYG_HUMAN antibody
    • Thyroglobulin antibody
    see all

Anti-Thyroglobulin antibody [EPR3614] images

  • Anti-Thyroglobulin antibody [EPR3614] (ab92467) at 1/10000 dilution + Human thyroid lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 305 kDa
  • ab92467, at a 1/2500 dilution, staining Thyroglobulin in paraffin embedded Human papillary thyroid carcinoma tissue by Immunohistochemisty.

References for Anti-Thyroglobulin antibody [EPR3614] (ab92467)

This product has been referenced in:
  • Fernando R  et al. Human fibrocytes coexpress thyroglobulin and thyrotropin receptor. Proc Natl Acad Sci U S A 109:7427-32 (2012). ICC/IF ; Human . Read more (PubMed: 22517745) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"