Primary antibody notesThis antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of Thyroglobulin in metastatic lesion establishes thyroid origin of tumor.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Use streptavidin biotin system or polymer system. Incubate 30 minutes at room temperature.
1/50 - 1/100.
Use a concentration of 10 - 20 µg/ml.
Incubate for 2 hours in the dark at room temperature, or alternatively incubate overnight at 4°C.
FunctionPrecursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Tissue specificityThyroid gland specific.
Involvement in diseaseDefects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases. Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family. Contains 11 thyroglobulin type-1 domains.
Post-translational modificationsSulfated tyrosines are desulfated during iodination.