Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)
Overview
- Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibodySee all Thyroid Hormone Receptor alpha 1+2 primary antibodies ...
- DescriptionRabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 246-295 of Human Thyroid Hormone Receptor alpha 1+2 (DQIILLKGCC MEIMSLRAAV RYDPESDTLT LSGEMAVKRE QLKNGGLGVV).
- Positive control
- Jurkat cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab105003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | Use a concentration of 0.5 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| IHC-P | Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionNuclear hormone receptor. High affinity receptor for triiodothyronine.
- Involvement in diseaseDefects in THRA are the cause of congenital hypothyroidism non-goitrous type 6 (CHNG6) [MIM:614450]. A disease characterized by growth retardation, developmental retardation, skeletal dysplasia, borderline low thyroxine levels and high triiodothyronine levels. There is differential sensitivity to thyroid hormone action, with retention of hormone responsiveness in the hypothalamic pituitary axis and liver but skeletal, gastrointestinal, and myocardial resistance.
- Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. - DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Cellular localizationNucleus.
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Information by UniProt
Target information above from: UniProt accession P10827 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) . -
Database links
- Entrez Gene: 396251 Chicken
- Entrez Gene: 281531 Cow
- Entrez Gene: 403601 Dog
- Entrez Gene: 7067 Human
- Entrez Gene: 21833 Mouse
- Entrez Gene: 81812 Rat
- Entrez Gene: 30670 Zebrafish
- Omim: 190120 Human
- SwissProt: P04625 Chicken
- SwissProt: E2QZW9 Dog
- SwissProt: P10827 Human
- SwissProt: P63058 Mouse
- SwissProt: P63059 Rat
- SwissProt: Q28570 Sheep
- SwissProt: Q98867 Zebrafish
- Unigene: 724 Human
- Unigene: 265917 Mouse
- Unigene: 442648 Mouse
- Unigene: 11307 Rat
see all -
Alternative names
- c-erbA-1 antibody
- C-erbA-alpha antibody
- EAR-7 antibody
- ERBA 1 antibody
- ERBA antibody
- ERBA related 7 antibody
- Nuclear receptor subfamily 1 group A member 1 antibody
- THA_HUMAN antibody
- THRA antibody
- Thra1 antibody
- Thra2 antibody
- Thyroid hormone receptor alpha 1 antibody
- Thyroid hormone receptor alpha 2 antibody
- Thyroid hormone receptor alpha antibody
- TR alpha 1 antibody
- TR alpha 2 antibody
- Tra1 antibody
- Triiodothyronine receptor antibody
- V-erbA-related protein 7 antibody
see all
Anti-Thyroid Hormone Receptor alpha 1+2 antibody images
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Western blot - Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003) at 0.5 µg/ml + Jurkat cell lysate at 10 µg
Predicted band size : 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)ab105003, at 4µg/ml, staining Thyroid Hormone Receptor alpha 1 + 2, in Human squamous epithelial cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Magnification 400x.
References for Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)
This product has been referenced in:
- Kiss-Toth E et al. A TNF Variant that Associates with Susceptibility to Musculoskeletal Disease Modulates Thyroid Hormone Receptor Binding to Control Promoter Activation. PLoS One 8:e76034 (2013). Read more (PubMed: 24069456) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"