Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)

Overview

  • Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibody
    See all Thyroid Hormone Receptor alpha 1+2 primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • Tested applicationsWB, IHC-P, IHCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 246-295 of Human Thyroid Hormone Receptor alpha 1+2 (DQIILLKGCC MEIMSLRAAV RYDPESDTLT LSGEMAVKRE QLKNGGLGVV).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-Thyroid Hormone Receptor alpha 1+2 antibody images

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 55 kDa
  • ab105003, at 4µg/ml, staining Thyroid Hormone Receptor alpha 1 + 2, in Human squamous epithelial cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Magnification 400x.

References for Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)

This product has been referenced in:
  • Kiss-Toth E  et al. A TNF Variant that Associates with Susceptibility to Musculoskeletal Disease Modulates Thyroid Hormone Receptor Binding to Control Promoter Activation. PLoS One 8:e76034 (2013). Read more (PubMed: 24069456) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"