Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibody
See all Thyroid Hormone Receptor alpha 1+2 primary antibodies -
DescriptionRabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
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Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 246-295 of Human Thyroid Hormone Receptor alpha 1+2 (DQIILLKGCC MEIMSLRAAV RYDPESDTLT LSGEMAVKRE QLKNGGLGVV).
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Positive control
- Jurkat cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab105003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionIsoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Isoform Alpha-2: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action. -
Involvement in diseaseHypothyroidism, congenital, non-goitrous, 6
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 396251 Chicken
- Entrez Gene: 281531 Cow
- Entrez Gene: 403601 Dog
- Entrez Gene: 7067 Human
- Entrez Gene: 21833 Mouse
- Entrez Gene: 81812 Rat
- Entrez Gene: 30670 Zebrafish
- Omim: 190120 Human
see all -
Alternative names
- c-erbA-1 antibody
- C-erbA-alpha antibody
- EAR-7 antibody
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Images
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Western blot - Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab105003) at 0.5 µg/ml + Jurkat cell lysate at 10 µg
Predicted band size: 55 kDa
Gel concentration 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thyroid Hormone Receptor alpha 1+2 antibody (ab105003)ab105003, at 4µg/ml, staining Thyroid Hormone Receptor alpha 1 + 2, in Human squamous epithelial cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Magnification 400x.
References
This product has been referenced in:
- Kiss-Toth E et al. A TNF Variant that Associates with Susceptibility to Musculoskeletal Disease Modulates Thyroid Hormone Receptor Binding to Control Promoter Activation. PLoS One 8:e76034 (2013). Read more (PubMed: 24069456) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"