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GDETQWPNGVKRKRKNSQCS, corresponding to amino acids 13-32 of Human Thyroid Hormone Receptor alpha 1.
Thyroid receptors have expression patterns that vary according to tissue and developmental stage. Almost all tissues express the alpha-1, alpha-2 and beta-1 isoforms, but beta-2 is synthesized almost exclusively in hypothalamus, anterior pituitary and developing ear. Receptor alpha-1 is the first isoform expressed in the conceptus.
Our Abpromise guarantee covers the use of ab15543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ab15543 staining Thyroid Hormone Receptor alpha 1 in colon carcinoma at 1/100 dilution by Immunohistochemistry (formalin/paraffin).
ab15543 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"