Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

Overview

  • Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibodySee all Thyroid Hormone Receptor alpha 1+2 primary antibodies ...
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • Tested applicationsWB, IHC-P, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A peptide corresponding to a region of Human THRA

  • Positive control
    • Jurkat cell lysate and human skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

Target

  • FunctionNuclear hormone receptor. High affinity receptor for triiodothyronine.
  • Involvement in diseaseDefects in THRA are the cause of congenital hypothyroidism non-goitrous type 6 (CHNG6) [MIM:614450]. A disease characterized by growth retardation, developmental retardation, skeletal dysplasia, borderline low thyroxine levels and high triiodothyronine levels. There is differential sensitivity to thyroid hormone action, with retention of hormone responsiveness in the hypothalamic pituitary axis and liver but skeletal, gastrointestinal, and myocardial resistance.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P10827 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AR7 antibody
    • Avian erythroblastic leukemia viral (v erb a) oncogene homolog antibody
    • c ERBA 1 antibody
    • c ERBA ALPHA 2 antibody
    • C erbA alpha antibody
    • c-erbA-1 antibody
    • C-erbA-alpha antibody
    • cerbA protein antibody
    • cerbAalpha antibody
    • EAR 7 antibody
    • EAR 7.1 antibody
    • EAR 7.2 antibody
    • EAR-7 antibody
    • EAR7 antibody
    • ERB T 1 antibody
    • ERBA 1 antibody
    • ERBA ALPHA antibody
    • ERBA antibody
    • ERBA related 7 antibody
    • ERBA1 antibody
    • Erythroblastic leukemia viral (v erb a) oncogene homolog avian antibody
    • MGC000261 antibody
    • MGC43240 antibody
    • NR1A 1 antibody
    • Nr1a1 antibody
    • Nuclear receptor subfamily 1 group A member 1 antibody
    • Oncogene ERBA antibody
    • THA_HUMAN antibody
    • THRA antibody
    • Thra1 antibody
    • Thra2 antibody
    • Thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (verba) oncogene homolog 1 formerly ERBA1) antibody
    • Thyroid hormone receptor alpha 1 antibody
    • Thyroid hormone receptor alpha 2 antibody
    • Thyroid hormone receptor alpha antibody
    • TR alpha 1 antibody
    • TR alpha 2 antibody
    • Tra1 antibody
    • Triiodothyronine receptor antibody
    • V-erbA-related protein 7 antibody
    see all

Anti-Thyroid Hormone Receptor alpha 1+2 antibody images

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

References for Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

ab42565 has not yet been referenced specifically in any publications.

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Expiration date: November 16th, 2012

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human TRa expressed in CHO-K1)
Specification Human TRa expressed in CHO-K1
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"