Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

Overview

  • Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibody
    See all Thyroid Hormone Receptor alpha 1+2 primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A peptide corresponding to a region of Human THRA

  • Positive control
    • Jurkat cell lysate and human skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

Target

Anti-Thyroid Hormone Receptor alpha 1+2 antibody images

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

References for Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

ab42565 has not yet been referenced specifically in any publications.

Product Wall



DISCOUNT CODE: ***
Expiration date: November 16th, 2012

I am very pleased to hear you would like to accept our offer and test ab42565 in ChIP. This code will give you: 1 freeprimary antibody before the expiration date. To redee...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human TRa expressed in CHO-K1)
Specification Human TRa expressed in CHO-K1
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"