Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

Overview

  • Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibody
    See all Thyroid Hormone Receptor alpha 1+2 primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • Tested applicationsWB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A peptide corresponding to a region of Human THRA

  • Positive control
    • Jurkat cell lysate and human skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

Target

  • FunctionNuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
  • Involvement in diseaseHypothyroidism, congenital, non-goitrous, 6
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AR7 antibody
    • Avian erythroblastic leukemia viral (v erb a) oncogene homolog antibody
    • c ERBA 1 antibody
    • c ERBA ALPHA 2 antibody
    • C erbA alpha antibody
    • c-erbA-1 antibody
    • C-erbA-alpha antibody
    • cerbA protein antibody
    • cerbAalpha antibody
    • EAR 7 antibody
    • EAR 7.1 antibody
    • EAR 7.2 antibody
    • EAR-7 antibody
    • EAR7 antibody
    • ERB T 1 antibody
    • ERBA 1 antibody
    • ERBA ALPHA antibody
    • ERBA antibody
    • ERBA related 7 antibody
    • ERBA1 antibody
    • Erythroblastic leukemia viral (v erb a) oncogene homolog avian antibody
    • MGC000261 antibody
    • MGC43240 antibody
    • NR1A 1 antibody
    • Nr1a1 antibody
    • Nuclear receptor subfamily 1 group A member 1 antibody
    • Oncogene ERBA antibody
    • THA_HUMAN antibody
    • THRA antibody
    • Thra1 antibody
    • Thra2 antibody
    • Thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (verba) oncogene homolog 1 formerly ERBA1) antibody
    • Thyroid hormone receptor alpha 1 antibody
    • Thyroid hormone receptor alpha 2 antibody
    • Thyroid hormone receptor alpha antibody
    • TR alpha 1 antibody
    • TR alpha 2 antibody
    • Tra1 antibody
    • Triiodothyronine receptor antibody
    • V-erbA-related protein 7 antibody
    see all

Anti-Thyroid Hormone Receptor alpha 1+2 antibody images

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

References for Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

ab42565 has not yet been referenced specifically in any publications.

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Expiration date: November 16th, 2012

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human TRa expressed in CHO-K1)
Specification Human TRa expressed in CHO-K1
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"