Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565)

Overview

  • Product name
    Anti-Thyroid Hormone Receptor alpha 1+2 antibody
    See all Thyroid Hormone Receptor alpha 1+2 primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A peptide corresponding to a region of Human THRA

  • Positive control
    • Jurkat cell lysate and human skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

ICC/IF 1/100.

Target

Images

  • Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58, 47 kDa
    Observed band size : 47,58 kDa
  • ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

References

ab42565 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



DISCOUNT CODE: ***
Expiration date: November 16th, 2012

I am very pleased to hear you would like to accept our offer and test ab42565 in ChIP. This code will give you: 1 freeprimary antibody before the expiration date. To redee...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human TRa expressed in CHO-K1)
Specification
Human TRa expressed in CHO-K1
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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