Anti-Thyroid Hormone Receptor alpha antibody (ab53729)

Overview

  • Product name
    Anti-Thyroid Hormone Receptor alpha antibody
    See all Thyroid Hormone Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor alpha
  • Specificity
    ab53729 detects endogenous levels of total Thyroid Hormone Receptor alpha 1 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human Thyroid Hormone Receptor alpha.

  • Positive control
    • Human colon carcinoma tissue; SKOV3 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
  • Involvement in disease
    Hypothyroidism, congenital, non-goitrous, 6
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AR7 antibody
    • c-erbA-1 antibody
    • C-erbA-alpha antibody
    • CHNG6 antibody
    • EAR-7 antibody
    • EAR7 antibody
    • ERB-T-1 antibody
    • ERBA antibody
    • ERBA-related 7 antibody
    • ERBA1 antibody
    • NR1A1 antibody
    • Nuclear receptor subfamily 1 group A member 1 antibody
    • THA_HUMAN antibody
    • THRA antibody
    • THRA1 antibody
    • THRA2 antibody
    • Thyroid hormone receptor alpha antibody
    • thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) antibody
    • thyroid normone nuclear receptor alpha variant 1 antibody
    • triiodothyronine receptor antibody
    • V-erbA-related protein 7 antibody
    see all

Anti-Thyroid Hormone Receptor alpha antibody images

  • ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with immunising peptide


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Thyroid Hormone Receptor alpha antibody (ab53729)

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Loading amount
50 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Brain)
Permeabilization
Yes - Triton X-100 in 10 % Donkey Serum
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mr. Yoshiyuki Henning

Verified customer

Submitted Sep 12 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Mouse Tissue lysate - whole (Kidney)
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Tissue lysate - whole (Kidney)
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Rat Tissue lysate - whole (Kidney)
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Fetal Bovine serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - Triton-X
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Feb 26 2014

Thank you for contacting us.

The immunogen sequence for ab53729 is from xxxxxx and shares 23% sequence homology to the beta isoform of the thyroid hormone receptor therefore this antibody is unlikely to recognise the beta isoform. See attach...

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Thank you for contacting us.

Please find attached the IHC-P protocol we have used.

In our experience this antibody would work with any general IHC-P protocol.

I hope this information is helpful to you. Please do not hesitate ...

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Thank you for contacting Abcam regarding our Thyroid Hormone Receptor Alpha antibodies.


Regarding ab53729, I have confirmed that this antibody will react with alpha-1, -2, -3, and -4 and is NOT specific for alpha 1. The immunogen range is...

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Thank you for your enquiry. I am sorry to hear you did not recieve a reply to your previous email to mailto:orders@abcam.com. I have checked the system and am not sure why this was not responded to and I apologise for the inconvenience.

I un...

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