Anti-Thyroid Hormone Receptor antibody [C3] - ChIP Grade (ab2743)

Overview

  • Product nameAnti-Thyroid Hormone Receptor antibody [C3] - ChIP Grade
    See all Thyroid Hormone Receptor primary antibodies
  • Description
    Mouse monoclonal [C3] to Thyroid Hormone Receptor - ChIP Grade
  • Tested applicationsSuitable for: ChIP, ICC/IF, IHC-P, Gel supershift assays, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Bird, Non Human Primates, Amphibians
  • Immunogen

    Other Immunogen Type corresponding to Human Thyroid Hormone Receptor aa 201-456.

  • EpitopeThis antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
  • Concentration information loading...
  • Primary antibody notesThyroid hormone receptors (TR) are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.
  • ClonalityMonoclonal
  • Clone numberC3
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF 1/20.
IHC-P 1/10 - 1/100.
Gel supershift assays Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).

Target

  • RelevanceThyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 triiodo L thyronine (T3). Thyroid hormones, through their interaction with the TR affect metabolic processes, growth and development.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ERBA ALPHA antibody
    • ERBA beta antibody
    • ERBA1 antibody
    • ERBA2 antibody
    • NR1A1 antibody
    • NR1A2 antibody
    • THR1 antibody
    • THRA antibody
    • THRA1 antibody
    • THRA2 antibody
    • THRB antibody
    • THRB1 antibody
    • THRB2 antibody
    • Thyroid hormone receptor alpha antibody
    • Thyroid hormone receptor beta antibody
    see all

Anti-Thyroid Hormone Receptor antibody [C3] - ChIP Grade images

  • Immunocytochemistry/Immunofluorescence analysis of Thyroid Hormone Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2743 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of Thyroid Hormone Receptor in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2743 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human thyroid tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with ab2743 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.



  • Predicted band size : 52 kDa

    Western blot of human thyroid hormone receptor using ab2743.

References for Anti-Thyroid Hormone Receptor antibody [C3] - ChIP Grade (ab2743)

This product has been referenced in:
  • Bhat MK  et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine. Biochem Biophys Res Commun 195:385-92 (1993). Read more (PubMed: 8363616) »

See 1 Publication for this product

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Thank you for your enquiry. The concentration is ~8.0 mg/ml. Please do not hesitate to contact us if you need any further info.

Thank you for your email. Some suggestions for isotype controls suitable for this mouse IgG1 isotype antibody are: ab18437, ab18443, ab18448. To view all of our isotype controls, just select "Tags and Markers" from the product and then click on "Isotyp...

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Thank you for your enquiry. Unfortunately, we don't have the corresponding peptides for these antibodies. If you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"