Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

Overview

  • Product nameAnti-Thyroid Hormone Receptor beta antibody - ChIP GradeSee all Thyroid Hormone Receptor beta primary antibodies ...
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor beta - ChIP Grade
  • SpecificityThis antibody does not detect TR alpha-1 or TRv alpha-2.
  • Tested applicationsICC/IF, Gel supershift assays, ELISA, ChIP, IHC-P, ICC, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81. With an N-terminal added cysteine.
    Sequence: IFHLDHDDVNDQSVSSAQTF

Properties

Applications

Our Abpromise guarantee covers the use of ab5622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/50 - 1/500.
Gel supershift assays Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
ICC 1/50 - 1/500.
WB 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).

representing recombinant human TRv beta-1 expressed in E. coli

Target

  • FunctionHigh affinity receptor for triiodothyronine.
  • Involvement in diseaseDefects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
    Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P10828 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
    • C ERBA 2 antibody
    • C ERBA BETA antibody
    • c-erbA-2 antibody
    • c-erbA-beta antibody
    • ERBA 2 antibody
    • ERBA BETA antibody
    • ERBA2 antibody
    • Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian antibody
    • generalized resistance to thyroid hormone antibody
    • GRTH antibody
    • MGC126109 antibody
    • MGC126110 antibody
    • NR1A2 antibody
    • Nuclear receptor subfamily 1 group A member 2 antibody
    • Oncogene ERBA2 antibody
    • PRTH antibody
    • THB_HUMAN antibody
    • THR 1 antibody
    • THR1 antibody
    • THRB 1 antibody
    • THRB 2 antibody
    • THRB antibody
    • THRB1 antibody
    • THRB2 antibody
    • Thyroid hormone nuclear receptor beta variant 1 antibody
    • Thyroid hormone receptor beta 1 antibody
    • Thyroid hormone receptor beta 2 antibody
    • Thyroid hormone receptor beta antibody
    • Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) antibody
    see all

Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade images

  • ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

References for Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

This product has been referenced in:
  • Modesti PA  et al. Reduced expression of thyroid hormone receptors and beta-adrenergic receptors in human failing cardiomyocytes. Biochem Pharmacol 75:900-6 (2008). Read more (PubMed: 18031713) »
  • Sun Y  et al. Liver proteome analysis of adaptive response in rat immediately after partial hepatectomy. Proteomics 7:4398-407 (2007). Read more (PubMed: 17979177) »

See all 2 Publications for this product

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