Anti-Thyroid Peroxidase antibody (ab123805)

Overview

  • Product nameAnti-Thyroid Peroxidase antibody
    See all Thyroid Peroxidase primary antibodies
  • Description
    Goat polyclonal to Thyroid Peroxidase
  • Specificityab123805 is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    ELRCGKHQAVGTSPQ

    , corresponding to C terminal amino acids 893-907 of Human Thyroid Peroxidase (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).

  • Positive control
    • Human thyroid gland lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab123805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 103 kDa.

Target

  • FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • PathwayHormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localizationMembrane and Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Anti-Thyroid Peroxidase antibody images

  • ab123805 (5µg/ml) staining of paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured staining in the cytoplasm of epithelial cells.

  • Anti-Thyroid Peroxidase antibody (ab123805) at 0.1 µg/ml + Thyroid Gland lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 103 kDa

References for Anti-Thyroid Peroxidase antibody (ab123805)

ab123805 has not yet been referenced specifically in any publications.

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