Overview

  • Product nameAnti-Thyroid Peroxidase antibody
    See all Thyroid Peroxidase primary antibodies
  • Description
    Mouse monoclonal to Thyroid Peroxidase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 672-780 of Human Thyroid Peroxidase with a 26kDa tag.

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Thyroid Peroxidase is approximately 1ng/ml when used as a capture antibody.

Target

  • FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • PathwayHormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localizationMembrane and Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Anti-Thyroid Peroxidase antibody images

  • Anti-Thyroid Peroxidase antibody (ab76935) at 5 µg/ml + Immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Thyroid Peroxidase antibody (ab76935) at 5 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : 103 kDa
  • Capture ELISA using ab76935 at 10ug/ml.

References for Anti-Thyroid Peroxidase antibody (ab76935)

ab76935 has not yet been referenced specifically in any publications.

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