Anti-Thyroid Peroxidase antibody [EPR5380] (ab109383)

Overview

  • Product nameAnti-Thyroid Peroxidase antibody [EPR5380]
    See all Thyroid Peroxidase primary antibodies
  • Description
    Rabbit monoclonal [EPR5380] to Thyroid Peroxidase
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C terminal of Human Thyroid Peroxidase

  • Positive control
    • WB: Human thyroid lysate IHC-P: Human thyroid gland tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 103 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Target

  • FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • PathwayHormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localizationMembrane and Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Anti-Thyroid Peroxidase antibody [EPR5380] images

  • Anti-Thyroid Peroxidase antibody [EPR5380] (ab109383) at 1/1000 dilution + Human thyroid lysate at 10 µg

    Predicted band size : 103 kDa
  • ab109383, at a 1/100 dilution, staining Thyroid Peroxidase in paraffin-embedded Human thyroid gland tissue

References for Anti-Thyroid Peroxidase antibody [EPR5380] (ab109383)

ab109383 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Human Tissue (Thyroid)
Specification Thyroid
Username

Miss. Juri Na

Verified customer

Submitted May 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"