Anti-Thyrotropin Releasing Hormone (TRH) antibody (ab87764)

Overview

  • Product nameAnti-Thyrotropin Releasing Hormone (TRH) antibody
    See all Thyrotropin Releasing Hormone (TRH) primary antibodies
  • Description
    Rabbit polyclonal to Thyrotropin Releasing Hormone (TRH)
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken, Human
  • Immunogen

    Synthetic Thyrotropin Releasing Hormone (TRH).

  • Positive control
    • Hypothalamus
  • General notes


    Dilute the antibody immediately before use with PBS or Tris.

Properties

Applications

Our Abpromise guarantee covers the use of ab87764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/400.
IHC-Fr 1/100 - 1/400.

Target

  • RelevanceThyrotropin releasing hormone (TRH) is a tripeptide hormone that stimulates the release of thyroid stimulating hormone and prolactin by the anterior pituitary. TRH is produced by the hypothalamus and travels across the median eminence to the pituitary via the hypophyseal portal system. In addition to the brain, TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Prothyroliberin antibody
    • Protirelin antibody
    • Thyroliberin [Precursor] antibody
    • Thyroliberin antibody
    • Thyrotropin releasing factor antibody
    • Thyrotropin-releasing hormone antibody
    • TRF antibody
    • TRH antibody
    • TSH releasing factor antibody
    see all

Anti-Thyrotropin Releasing Hormone (TRH) antibody images

  • ab87764 staining Thyrotropin Releasing Hormone (TRH) in chicken brain sections by Immunohistochemistry (Frozen sections).
    The tissue was fixed in paraformaldehyde, permeabilized using PBS/ 0.1% Triton X-100, blocked with 4% BSA, 5% normal goat serum, 0.1M lysine and 0.1% Triton X-100 in PBS and then incubated with ab87764 at a 1/100 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    Antigen retrieval was performed with a Tris-EDTA buffer, pH 9 (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween 20, pH 9.0).

    See Abreview

References for Anti-Thyrotropin Releasing Hormone (TRH) antibody (ab87764)

ab87764 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Chicken brain cryosections)
Specification Chicken brain cryosections
Fixative Paraformaldehyde
Permeabilization Yes - PBS-0.1% Triton X-100
Blocking step 4% BSA, 5% Normal Goat Serum, 0.1M lysine and 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Apr 02 2012

Thank you for your contacting us. I confirm the immunogen used for developing the anti-TRH antibody consisted of a synthetic TRH tripeptide and not of the prohormone.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"