Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TIA1.
(Peptide available as ab41475.)
Our Abpromise guarantee covers the use of ab40693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab40693 staining TIA1 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody. The image shows clean predominantly nuclear staining.