The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1:128,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.