Recombinant
RabMAb

Anti-TIA1 antibody [EPR9304] (HRP) (ab195526)

Overview

  • Product name
    Anti-TIA1 antibody [EPR9304] (HRP)
    See all TIA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9304] to TIA1 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TIA1 aa 350 to the C-terminus (C terminal).

  • Positive control
    • WB: Jurkat, MOLT4 and K562 whole cell lysates. IHC-P: normal human spleen.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-TIA1 antibody [EPR9304] (ab140595)

    Anti-TIA1 antibody (Alexa Fluor® 647) [EPR9304] (ab196170)
    Anti-TIA1 antibody (Alexa Fluor® 488) [EPR9304] (ab196382)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab195526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization
    Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytotoxic granule associated RNA binding protein 1 antibody
    • Cytotoxic granule associated RNA binding protein antibody
    • mTIA-1 antibody
    • Nucleolysin TIA 1 isoform p40 antibody
    • Nucleolysin TIA-1 isoform p40 antibody
    • Nucleolysin TIA1 isoform p40 antibody
    • p40 TIA 1 antibody
    • p40-TIA-1 (containing p15-TIA-1) antibody
    • p40-TIA-1 antibody
    • RNA binding protein TIA 1 antibody
    • RNA binding protein TIA1 antibody
    • RNA-binding protein TIA-1 antibody
    • T-cell-restricted intracellular antigen-1 antibody
    • TIA 1 antibody
    • TIA 1 cytotoxic granule associated RNA binding protein antibody
    • Tia antibody
    • TIA-1 antibody
    • TIA1 antibody
    • TIA1 cytotoxic granule associated RNA binding protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 protein antibody
    • TIA1_HUMAN antibody
    • TIAL1 antibody
    • TIAR antibody
    • WDM antibody
    see all

Images

  • All lanes : Anti-TIA1 antibody [EPR9304] (HRP) (ab195526) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of TIA1 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195526 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab195526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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