Overview

  • Product nameAnti-TIA1 antibody [TIA-1]
    See all TIA1 primary antibodies
  • Description
    Mouse monoclonal [TIA-1] to TIA1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Positive control
    • Anaplastic large cell lymphoma or tonsil.
  • General notes


    TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Primary antibody notesTIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.
  • ClonalityMonoclonal
  • Clone numberTIA-1
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

Target

  • FunctionInvolved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
  • Cellular localizationCytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytotoxic granule associated RNA binding protein 1 antibody
    • Cytotoxic granule associated RNA binding protein antibody
    • mTIA-1 antibody
    • Nucleolysin TIA 1 isoform p40 antibody
    • Nucleolysin TIA-1 isoform p40 antibody
    • Nucleolysin TIA1 isoform p40 antibody
    • p40 TIA 1 antibody
    • p40-TIA-1 (containing p15-TIA-1) antibody
    • p40-TIA-1 antibody
    • RNA binding protein TIA 1 antibody
    • RNA binding protein TIA1 antibody
    • RNA-binding protein TIA-1 antibody
    • T-cell-restricted intracellular antigen-1 antibody
    • TIA 1 antibody
    • TIA 1 cytotoxic granule associated RNA binding protein antibody
    • Tia antibody
    • TIA-1 antibody
    • TIA1 antibody
    • TIA1 cytotoxic granule associated RNA binding protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 protein antibody
    • TIA1_HUMAN antibody
    • TIAL1 antibody
    • TIAR antibody
    • WDM antibody
    see all

Anti-TIA1 antibody [TIA-1] images

  • ab2712 at 1/3300 dilution staining monkey COS 7 cells by ICC/IF. The cells were cultured for 2 hours at 45oC and fixed in cold Methanol: Acetone 1:1 for 10 minutes. The TIA1 antibody was directly conjugated with Alexa Fluor ® 568 and after 1 hour incubation a second fixation step was performed.

    See Abreview

  • Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-TIA1 antibody [TIA-1] (ab2712)

This product has been referenced in:
  • Subbiah V  et al. A novel immunomodulatory molecularly targeted strategy for refractory Hodgkin's lymphoma. Oncotarget 5:95-102 (2014). IHC . Read more (PubMed: 24395633) »
  • Rodriguez-Ortiz CJ  et al. Neuronal-specific overexpression of a mutant valosin-containing protein associated with IBMPFD promotes aberrant ubiquitin and TDP-43 accumulation and cognitive dysfunction in transgenic mice. Am J Pathol 183:504-15 (2013). Mouse . Read more (PubMed: 23747512) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.We have not teted the specificity, the only information we have available is that the TIA-1 antibody reaacts with a 15 kDa cytoplasmic granule-associated protein. Please let me know if you have any further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (neuroblastoma)
Specification neuroblastoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X 100
Blocking step goat serum,0.5%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Verified customer

Submitted Oct 02 2012

Thank you very much for your email.

One vial of ab2712 has been added to your order, and you can pay for this order with the PO that you provided or with a credit card if you prefer. If you'd like to add a credit card to your order, please ca...

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Expiration date: January 5th, 2012

I am very pleased to hear you would like to accept our offer and test ab2712 in ICC. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer...

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Application Western blot
Sample Spermophilus tridecemlineatus Tissue lysate - whole (Liver, Heart)
Loading amount 30 µg
Specification Liver, Heart
Gel Running Conditions Reduced Denaturing (15%)
Blocking step PVA as blocking agent for 1 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 06 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Renal proximal tubular epithelial cells)
Specification Renal proximal tubular epithelial cells
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Robert Jenkins

Verified customer

Submitted Apr 13 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS 7 cells)
Specification COS 7 cells
Fixative Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3
Username

Mrs. Gabriela Elena Oprea

Verified customer

Submitted Aug 16 2006

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primary cultured hippocampal neurons, COS)
Specification primary cultured hippocampal neurons, COS
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Jun 05 2006

Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for ...

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Thank you for taking the time to submit a review to our system. I am taking your comments seriously and would appreciate your assistance to enable me to look into the source of the problem in more detail. Could you fill out the questionnaire at the f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"