Anti-TIAL1 antibody (ab129499)
Key features and details
- Rabbit polyclonal to TIAL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIAL1 antibody
See all TIAL1 primary antibodies -
Description
Rabbit polyclonal to TIAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 325-375 of Human TIAL1 (NP_003243.1).
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Positive control
- HeLa, 293T, and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 771327 Chicken
- Entrez Gene: 614540 Cow
- Entrez Gene: 477841 Dog
- Entrez Gene: 101143159 Gorilla
- Entrez Gene: 100058604 Horse
- Entrez Gene: 7073 Human
- Entrez Gene: 21843 Mouse
- Entrez Gene: 100158186 Pig
see all -
Alternative names
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
see all
Images
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All lanes : Anti-TIAL1 antibody (ab129499) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds -
Detection of TIAL1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab129499 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129499 has been referenced in 1 publication.
- Wang Q et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). PubMed: 30569136