Overview

  • Product name
    Anti-TIAL1 + TIA antibody
  • Description
    Goat polyclonal to TIAL1 + TIA
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Peptide with sequence

    C-SPEITTEDIKSAFAP

    , corresponding to amino acids 105-119 from the internal region of the TIAL1 + TIA protein (NP_003243.1; NP_001029097.1).

  • Positive control
    • K562 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.
  • Cellular localization
    Cytoplasmic granule
  • Database links
  • Alternative names
    • MGC33401 antibody
    • T cluster binding protein antibody
    • TCBP antibody
    • TIA 1 related nucleolysin antibody
    • TIA 1 related protein antibody
    • TIA1 cytotoxic granule associated RNA binding p antibody
    • TIAR antibody
    see all

Images

  • ab105032 (3.8µg/ml) staining of paraffin embedded Human Skin following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of select keratinocytes.

  • Anti-TIAL1 + TIA antibody (ab105032) at 2 µg/ml + K562 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 1 hour

References

ab105032 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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