Overview

  • Product nameAnti-TIAM2 antibody
    See all TIAM2 primary antibodies
  • Description
    Mouse monoclonal to TIAM2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: DVHPEAEQQP GPESGEGQKG GEQPKLVRGH FCPIKRKANS TKRDRGTLLK AQIRHQSLDS QSENATIDLN SVLEREFSVQ SLTSVVSEEC FYETESHG, corresponding to amino acids 527-625 of Human TIAM2

Properties

Applications

Our Abpromise guarantee covers the use of ab57033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionModulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
    • Tissue specificityExpressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
    • Sequence similaritiesBelongs to the TIAM family.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PDZ (DHR) domain.
      Contains 2 PH domains.
      Contains 1 RBD (Ras-binding) domain.
    • DomainThe PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth.
    • Cellular localizationCytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ41865 antibody
      • OTTHUMP00000017472 antibody
      • SIF and TIAM1 like exchange factor antibody
      • SIF and TIAM1-like exchange factor antibody
      • STEF antibody
      • T cell lymphoma invasion and metastasis 2 antibody
      • T lymphoma invasion and metastasis inducing protein 2 antibody
      • T-lymphoma invasion and metastasis-inducing protein 2 antibody
      • TIAM 2 antibody
      • TIAM-2 antibody
      • Tiam2 antibody
      • TIAM2_HUMAN antibody
      see all

    Anti-TIAM2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57033 TIAM2 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-TIAM2 antibody (ab57033)

    ab57033 has not yet been referenced specifically in any publications.

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