Overview

  • Product nameAnti-TIAM2 antibody
    See all TIAM2 primary antibodies
  • Description
    Rabbit polyclonal to TIAM2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TIAM2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse hippocampus; Mouse cerebellum; Rat cortex; Rat olfactory bulb.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 193 kDa).

Target

  • FunctionModulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.
  • Tissue specificityExpressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
  • Sequence similaritiesBelongs to the TIAM family.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 RBD (Ras-binding) domain.
  • DomainThe PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth.
  • Cellular localizationCytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ41865 antibody
    • OTTHUMP00000017472 antibody
    • SIF and TIAM1 like exchange factor antibody
    • SIF and TIAM1-like exchange factor antibody
    • STEF antibody
    • T cell lymphoma invasion and metastasis 2 antibody
    • T lymphoma invasion and metastasis inducing protein 2 antibody
    • T-lymphoma invasion and metastasis-inducing protein 2 antibody
    • TIAM 2 antibody
    • TIAM-2 antibody
    • Tiam2 antibody
    • TIAM2_HUMAN antibody
    see all

Anti-TIAM2 antibody images

  • All lanes : Anti-TIAM2 antibody (ab93839) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate
    Lane 5 : Rat Olfactory Bulb Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-TIAM2 antibody (ab93839)

ab93839 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (3-8%)
Sample Human Cell lysate - whole cell (Hek293, U2OS, HepG2)
Specification Hek293, U2OS, HepG2
Blocking step Caesin blocking buffer (Sigma) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Aishwarya Payapilly

Verified customer

Submitted Apr 10 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - 0.1% Triton-X-100
Fixative Formaldehyde
Username

Dr. Aishwarya Payapilly

Verified customer

Submitted Apr 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"