Anti-TICAM2 antibody [mAbcam77169] (ab77169)
Key features and details
- Mouse monoclonal [mAbcam77169] to TICAM2
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TICAM2 antibody [mAbcam77169]
See all TICAM2 primary antibodies -
Description
Mouse monoclonal [mAbcam77169] to TICAM2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TICAM2 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab57980) -
Positive control
- Recombinant Human TICAM2 protein (ab123160) can be used as a positive control in WB. This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam77169 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. -
Tissue specificity
Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas. -
Sequence similarities
Contains 1 TIR domain. -
Domain
The TIR domain mediates the interaction with TRAF6. -
Post-translational
modificationsPhosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling. -
Cellular localization
Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. - Information by UniProt
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Database links
- Entrez Gene: 100302736 Human
- Entrez Gene: 353376 Human
- Omim: 608321 Human
- SwissProt: Q86XR7 Human
- Unigene: 709120 Human
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Alternative names
- Putative NF-kappa-B-activating protein 502 antibody
- TCAM2_HUMAN antibody
- TICAM-2 antibody
see all
Images
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All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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Datasheet download
References (0)
ab77169 has not yet been referenced specifically in any publications.