Anti-TICAM2 antibody [mAbcam77169] (ab77169)

Overview

  • Product nameAnti-TICAM2 antibody [mAbcam77169]
    See all TICAM2 primary antibodies
  • Description
    Mouse monoclonal [mAbcam77169] to TICAM2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TICAM2.

    (Peptide available as ab57980.)

  • Positive control
    • This antibody gave a positive signal in Human Kidney and Human Spleen Tissue Lyates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numbermAbcam77169
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionFunctions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.
  • Tissue specificityExpressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.
  • Sequence similaritiesContains 1 TIR domain.
  • DomainThe TIR domain mediates the interaction with TRAF6.
  • Post-translational
    modifications
    Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
    Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
  • Cellular localizationCytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Putative NF-kappa-B-activating protein 502 antibody
    • TCAM2_HUMAN antibody
    • TICAM-2 antibody
    • TICAM2 antibody
    • TIR containing adapter molecule 2 antibody
    • TIR domain containing adaptor protein antibody
    • TIR domain-containing adapter molecule 2 antibody
    • TIRAP 3 antibody
    • TIRAP3 antibody
    • TIRAP3a short form antibody
    • TIRP antibody
    • Toll-like receptor adaptor protein 3 antibody
    • Toll/interleukin-1 receptor domain-containing protein antibody
    • TRAM antibody
    • TRIF-related adapter molecule antibody
    see all

Anti-TICAM2 antibody [mAbcam77169] images

  • All lanes : Anti-TICAM2 antibody [mAbcam77169] (ab77169) at 10 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing PC3 cells stained with ab77169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77169, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TICAM2 antibody [mAbcam77169] (ab77169)

ab77169 has not yet been referenced specifically in any publications.

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