Recombinant
RabMAb

Anti-TID1 antibody [EPR12414] (ab181024)

Overview

  • Product name
    Anti-TID1 antibody [EPR12414]
    See all TID1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12414] to TID1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TID1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96EY1

  • Positive control
    • JAR, Jurkat, HeLa and K562 cell lysate. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/250.
IP 1/30.

Target

  • Relevance
    TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
  • Cellular localization
    Mitochondrial
  • Database links
  • Alternative names
    • DnaJ (Hsp40) homolog subfamily A member 3 antibody
    • DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
    • DnaJ protein Tid 1 antibody
    • DnaJ protein Tid1 antibody
    • FLJ45758 antibody
    • HCA57 antibody
    • Hepatocellular carcinoma associated antigen 57 antibody
    • Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME antibody
    • hTid 1 antibody
    • hTid1 antibody
    • III [Caenorhabditis elegans] antibody
    • TID1 antibody
    • Tumorous imaginal discs (Drosophila) homolog antibody
    • Tumorous imaginal discs protein Tid56 homolog antibody
    see all

Images

  • All lanes : Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

  • Lysate from Jurkat cells  (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

References

ab181024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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