Overview

  • Product nameAnti-TID1 antibody [RS13]
    See all TID1 primary antibodies
  • Description
    Mouse monoclonal [RS13] to TID1
  • SpecificityReacts with both short and long variants of TID1.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human TID1 protein.

  • Positive control
    • HeLa and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab81700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 µg/mg of lysate. Co precipitates mitochondrial Hsp70.
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
    • Cellular localizationMitochondrial
    • Database links
    • Alternative names
      • DnaJ (Hsp40) homolog subfamily A member 3 antibody
      • DnaJ homolog subfamily A member 3 mitochondrial precursor antibody
      • DnaJ protein Tid 1 antibody
      • DnaJ protein Tid1 antibody
      • FLJ45758 antibody
      • HCA57 antibody
      • Hepatocellular carcinoma associated antigen 57 antibody
      • Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME antibody
      • hTid 1 antibody
      • hTid1 antibody
      • III [Caenorhabditis elegans] antibody
      • TID1 antibody
      • Tumorous imaginal discs (Drosophila) homolog antibody
      • Tumorous imaginal discs protein Tid56 homolog antibody
      see all

    Anti-TID1 antibody [RS13] images

    References for Anti-TID1 antibody [RS13] (ab81700)

    This product has been referenced in:
    • Proft J  et al. Identification of Bilateral Changes in TID1 Expression in the 6-OHDA Rat Model of Parkinson's Disease. PLoS One 6:e26045 (2011). WB, IHC-Fr ; Rat . Read more (PubMed: 22016808) »
    • Syken J  et al. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions. Proc Natl Acad Sci U S A 96:8499-504 (1999). Human . Read more (PubMed: 10411904) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (293T kidney cells)
    Total protein in input 150 µg
    Specification 293T kidney cells
    Treatment tranfected with Tid1-flag
    Immuno-precipitation step Protein G
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Dec 14 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (HCT116 colon cancer cell line)
    Loading amount 60 µg
    Specification HCT116 colon cancer cell line
    Treatment different treatments
    Gel Running Conditions Reduced Denaturing (13%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Christian Marx

    Verified customer

    Submitted Oct 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"