• Product name
    Anti-TIE1 antibody [8C9]
    See all TIE1 primary antibodies
  • Description
    Mouse monoclonal [8C9] to TIE1
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
    Database link: P35590



Our Abpromise guarantee covers the use of ab27851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
  • Tissue specificity
    Specifically expressed in developing vascular endothelial cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • JKT 14 antibody
    • JTK14 antibody
    • TIE antibody
    • Tie1 antibody
    • TIE1_HUMAN antibody
    • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
    • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
    • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
    • Tyrosine protein kinase receptor Tie 1 antibody
    • Tyrosine-protein kinase receptor Tie-1 antibody
    see all

Anti-TIE1 antibody [8C9] images

  • Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-TIE1 antibody [8C9] (ab27851)

    Lane 1 : Recombinant human soluble TIE1
    Lane 2 : Recombinant human soluble TIE2
    Lane 3 : Recombinant mouse soluble TIE1
    Lane 4 : Recombinant mouse soluble TIE2

    Predicted band size : 125 kDa

References for Anti-TIE1 antibody [8C9] (ab27851)

ab27851 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab27851.
Please use the links above to contact us or submit feedback about this product.


Sign up