Overview

  • Product nameAnti-TIE1 antibody [8C9]
    See all TIE1 primary antibodies
  • Description
    Mouse monoclonal [8C9] to TIE1
  • Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
    Database link: P35590

Properties

Applications

Our Abpromise guarantee covers the use of ab27851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTransmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
  • Tissue specificitySpecifically expressed in developing vascular endothelial cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • JKT 14 antibody
    • JTK14 antibody
    • TIE antibody
    • Tie1 antibody
    • TIE1_HUMAN antibody
    • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
    • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
    • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
    • Tyrosine protein kinase receptor Tie 1 antibody
    • Tyrosine-protein kinase receptor Tie-1 antibody
    see all

Anti-TIE1 antibody [8C9] images

  • Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-TIE1 antibody [8C9] (ab27851)

    Lane 1 : Recombinant human soluble TIE1
    Lane 2 : Recombinant human soluble TIE2
    Lane 3 : Recombinant mouse soluble TIE1
    Lane 4 : Recombinant mouse soluble TIE2


    Predicted band size : 125 kDa

References for Anti-TIE1 antibody [8C9] (ab27851)

ab27851 has not yet been referenced specifically in any publications.

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