Anti-TIE1 antibody [8D12D2] (ab201986)
Key features and details
- Mouse monoclonal [8D12D2] to TIE1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TIE1 antibody [8D12D2]
See all TIE1 primary antibodies -
Description
Mouse monoclonal [8D12D2] to TIE1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human TIE1 aa 350-650 (internal sequence).
Database link: P35590 -
Positive control
- Human TIE1 (AA: 385-607) recombinant protein, TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cell lysate, HepG2 cell lysate, Human ovarian cancer tissues, Human kidney tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D12D2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 125 kDa.
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Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 125 kDa. |
Target
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Function
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. -
Tissue specificity
Specifically expressed in developing vascular endothelial cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 280941 Cow
- Entrez Gene: 7075 Human
- Entrez Gene: 21846 Mouse
- Omim: 600222 Human
- SwissProt: Q06805 Cow
- SwissProt: P35590 Human
- SwissProt: Q06806 Mouse
- Unigene: 78824 Human
see all -
Alternative names
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TIE1 with ab201986 at 1/200 dilution with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling TIE1 with ab201986 at 1/200 dilution with DAB staining.
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Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 125 kDa -
All lanes : Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 125 kDa -
Anti-TIE1 antibody [8D12D2] (ab201986) at 1/500 dilution + Human TIE1 (AA: 385-607) recombinant protein
Predicted band size: 125 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab201986 has been referenced in 3 publications.
- Zhao J et al. Cell-fate transition and determination analysis of mouse male germ cells throughout development. Nat Commun 12:6839 (2021). PubMed: 34824237
- Li T et al. TIPE1 impairs ovarian tumor growth by promoting caspase-dependent apoptosis. Oncol Lett 20:365 (2020). PubMed: 33133265
- Xu M et al. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. Cell 178:1478-1492.e20 (2019). PubMed: 31474362