Anti-TIE1 antibody (ab64477)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TIE1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C terminus of human TIE1

  • Positive control
    • Human tonsil tissue
  • General notes
    Cell membrane staining pattern

Properties

Applications

Our Abpromise guarantee covers the use of ab64477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 for 30 min at room temperature.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
    • Tissue specificity
      Specifically expressed in developing vascular endothelial cells.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
      Contains 3 EGF-like domains.
      Contains 3 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • JKT 14 antibody
      • JTK14 antibody
      • TIE antibody
      • Tie1 antibody
      • TIE1_HUMAN antibody
      • Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody
      • Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains antibody
      • Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody
      • Tyrosine protein kinase receptor Tie 1 antibody
      • Tyrosine-protein kinase receptor Tie-1 antibody
      see all

    Images

    • ab64477, at a dilution of 1/100, staining TIE1 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunohistochemistry.

    References

    ab64477 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0, 96-99¯C, 20 min.
    Permeabilization
    No
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Formalin
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2016

    Thank you for your enquiry. Unfortunately, I am sorry we are not able to release the immunogen sequence for this particular antibody as it is considered commercially sensitive. However, our source for this antibody has confirmed that the sequen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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