Overview

  • Product nameAnti-TIE2 antibody [TEK4] (Biotin)
    See all TIE2 primary antibodies
  • Description
    Rat monoclonal [TEK4] to TIE2 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse TIE2 extracellular domain

  • Positive control
    • Mouse bone marrow cell suspensions

Properties

Applications

Our Abpromise guarantee covers the use of ab95721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105-8 cells. A final voulme of 100µl is recommended.



ab18403-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.
  • Tissue specificityDetected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • Involvement in diseaseDominantly inherited venous malformations
    May play a role in a range of diseases with a vascular component, including neovascularization of tumors, psoriasis and inflammation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • DomainThe soluble extracellular domain is functionally active in angiopoietin binding and can modulate the activity of the membrane-bound form by competing for angiopoietins.
  • Post-translational
    modifications
    Proteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2).
    Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB.
    Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
  • Cellular localizationCell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Angiopoietin 1 receptor antibody
    • Angiopoietin-1 receptor antibody
    • CD202b antibody
    • CD202b antigen antibody
    • Endothelial tyrosine kinase antibody
    • Endothelium specific receptor tyrosine kinase 2 antibody
    • hTIE 2 antibody
    • hTIE2 antibody
    • Hyk antibody
    • p140 TEK antibody
    • Soluble TIE2 variant 1 antibody
    • Soluble TIE2 variant 2 antibody
    • Tek antibody
    • tek tyrosine kinase antibody
    • TEK tyrosine kinase endothelial antibody
    • tek tyrosine kinase, endothelial antibody
    • TIE 2 antibody
    • TIE2 antibody
    • TIE2_HUMAN antibody
    • Tunica interna endothelial cell kinase antibody
    • Tyrosine kinase with Ig and EGF homology domains 2 antibody
    • Tyrosine kinase with Ig and EGF homology domains-2 antibody
    • Tyrosine protein kinase receptor TEK antibody
    • Tyrosine protein kinase receptor TIE 2 antibody
    • Tyrosine-protein kinase receptor TEK antibody
    • Tyrosine-protein kinase receptor TIE-2 antibody
    • Venous malformations multiple cutaneous and mucosal antibody
    • VMCM 1 antibody
    • VMCM antibody
    • VMCM1 antibody
    see all

References for Anti-TIE2 antibody [TEK4] (Biotin) (ab95721)

This product has been referenced in:
  • Lelievre E  et al. Deficiency in the p110alpha subunit of PI3K results in diminished Tie2 expression and Tie2(-/-)-like vascular defects in mice. Blood 105:3935-8 (2005). Read more (PubMed: 15687236) »
  • Hsu HC  et al. Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver. Blood 96:3757-62 (2000). Read more (PubMed: 11090057) »

See all 2 Publications for this product

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