Recombinant
RabMAb

Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)] (ab151704)

Overview

  • Product name
    Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)]
  • Description
    Rabbit monoclonal [EPR1053(N)(B)] to TIE2 + TIE1 (phospho Y992 + Y1007)
  • Host species
    Rabbit
  • Specificity
    ab151704 only detects TIE2 phosphorylated at tyrosine 992.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIE2 + TIE1 (phospho Y992). The exact sequence is proprietary.

  • Positive control
    • WB: HUVEC cell lysate treated with pervanadate. ICC/IF: HUVEC cells treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Images

    • All lanes : Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : HUVEC cell lysate treated with pervanadate
      Lane 2 : HUVEC cell lysate treated with pervanadate with TIE2 (phospho Y992) peptide
      Lane 3 : HUVEC cell lysate treated with pervanadate with TIE2 unmodified peptide
      Lane 4 : HUVEC cell lysate treated with pervanadate with TIE1 (phospho Y1007) peptide
      Lane 5 : HUVEC cell lysate treated with pervanadate with TIE1 unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 5 seconds


      Blocking buffer: 5% BSA/TBST
      Dilution buffer: 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody

    • Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

      Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

    • All lanes : Anti-TIE2 + TIE1 (phospho Y992 + Y1007) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : Untreated HUVEC whole cell lysates
      Lane 2 : HUVEC treated with Pervanadate whole cell lysates
      Lane 3 : HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 10 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

    • Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    References

    ab151704 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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