Overview

  • Product name
  • Description
    Rabbit polyclonal to TIF1 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 19 amino acids from an internal region of Human TIF1 alpha (NP_056989, Uniprot: O15164).

  • Positive control
    • Mouse EL4 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab167089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 117 kDa.

Target

  • Function
    Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
  • Sequence similarities
    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • Cellular localization
    Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase TRIM24 antibody
    • E3 ubiquitin-protein ligase Trim24 antibody
    • hTIF1 antibody
    • PTC6 antibody
    • RING finger protein 82 antibody
    • RNF82 antibody
    • TF1A antibody
    • TIF1 antibody
    • TIF1-alpha antibody
    • TIF1A antibody
    • TIF1A_HUMAN antibody
    • TIF1ALPHA antibody
    • Transcription intermediary factor 1-alpha antibody
    • Transcriptional intermediary factor 1 alpha antibody
    • Transcriptional intermediary factor 1 antibody
    • Trim24 antibody
    • Tripartite motif containing 24 antibody
    • Tripartite motif-containing protein 24 antibody
    see all

Images

  • Western Blot of A431 cell lysate labeling TIF1 alpha with Anti-TIF1 alpha antibody (ab167089) at (1) 0.5 and (2) 1µg/ml.
  • Lane 1 : Anti-TIF1 alpha antibody (ab167089) at 0.5 µg/ml
    Lane 2 : Anti-TIF1 alpha antibody (ab167089) at 1 µg/ml

    All lanes : mouse EL4 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 117 kDa

References

ab167089 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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