Anti-TIF1 alpha antibody (ab38264)

Overview

• Product name
  Anti-TIF1 alpha antibody
  See all TIF1 alpha primary antibodies
• Description
  Goat polyclonal to TIF1 alpha
• Specificity
  This antibody is expected to recognise both reported isoforms, as represented by NP_003843 and NP_056989.
• Tested applications
  Suitable for: WB, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide:

  KRLKSIEERQLLK

  , corresponding to C terminal amino acids 1038-1050 of Human TIF1 alpha

  Run BLAST with Run BLAST with

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris saline. pH 7.3
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

Applications

Target

• Function
  Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
• Pathway
  Protein modification; protein ubiquitination.
• Involvement in disease
  Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
• Sequence similarities
  Contains 2 B box-type zinc fingers.
  Contains 1 bromo domain.
  Contains 1 PHD-type zinc finger.
  Contains 1 RING-type zinc finger.
• Post-translational
  modifications
  Phosphorylated upon DNA damage, probably by ATM or ATR.
  Sumoylated.
• Cellular localization
  Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.

• Target information above from: UniProt accession O15164 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 8805 Human
  • Omim: 603406 Human
  • SwissProt: O15164 Human
  • Unigene: 490287 Human
• Alternative names
  • E3 ubiquitin protein ligase TRIM24 antibody
  • E3 ubiquitin-protein ligase Trim24 antibody
  • hTIF1 antibody

  • PTC6 antibody
  • RING finger protein 82 antibody
  • RNF82 antibody
  • TF1A antibody
  • TIF1 antibody
  • TIF1-alpha antibody
  • TIF1A antibody
  • TIF1A_HUMAN antibody
  • TIF1ALPHA antibody
  • Transcription intermediary factor 1-alpha antibody
  • Transcriptional intermediary factor 1 alpha antibody
  • Transcriptional intermediary factor 1 antibody
  • Trim24 antibody
  • Tripartite motif containing 24 antibody
  • Tripartite motif-containing protein 24 antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols