Overview

  • Product nameAnti-TIF1 alpha antibody
    See all TIF1 alpha primary antibodies
  • Description
    Goat polyclonal to TIF1 alpha
  • SpecificityThis antibody is expected to recognise both reported isoforms, as represented by NP_003843 and NP_056989.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    KRLKSIEERQLLK

    , corresponding to C terminal amino acids 1038-1050 of Human TIF1 alpha

Properties

Applications

Our Abpromise guarantee covers the use of ab38264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
  • Sequence similaritiesContains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • Cellular localizationNucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase TRIM24 antibody
    • E3 ubiquitin-protein ligase Trim24 antibody
    • hTIF1 antibody
    • PTC6 antibody
    • RING finger protein 82 antibody
    • RNF82 antibody
    • TF1A antibody
    • TIF1 antibody
    • TIF1-alpha antibody
    • TIF1A antibody
    • TIF1A_HUMAN antibody
    • TIF1ALPHA antibody
    • Transcription intermediary factor 1-alpha antibody
    • Transcriptional intermediary factor 1 alpha antibody
    • Transcriptional intermediary factor 1 antibody
    • Trim24 antibody
    • Tripartite motif containing 24 antibody
    • Tripartite motif-containing protein 24 antibody
    see all

Anti-TIF1 alpha antibody images

  • ab38264 (4µg/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows nuclear staining in the distorted lobules.

References for Anti-TIF1 alpha antibody (ab38264)

ab38264 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"