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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIF1 alpha aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: O15164
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab174287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 116 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TIF1 alpha knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab174287 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab174287 was shown to specifically react with TIF1 alpha when TIF1 alpha knockout samples were used. Wild-type and TIF1 alpha knockout samples were subjected to SDS-PAGE. ab174287 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TIF1-alpha with ab174287 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded paraffin-embedded Human endometrial carcinoma tissue labeling TIF1-alpha using ab174287 at 1/50 dilution.
ab174287 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"