Anti-TIF1 gamma antibody [16G9] (ab33475)


  • Product nameAnti-TIF1 gamma antibody [16G9]
    See all TIF1 gamma primary antibodies
  • Description
    Mouse monoclonal [16G9] to TIF1 gamma
  • Tested applicationsSuitable for: ICC, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human): TIF1 gamma.



Our Abpromise guarantee covers the use of ab33475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at a concentration of 4 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 123 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
    • Tissue specificityExpressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseDefects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.
    • Sequence similaritiesBelongs to the TRIM/RBCC family.
      Contains 2 B box-type zinc fingers.
      Contains 1 bromo domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 RING-type zinc finger.
    • Cellular localizationNucleus. In discrete nuclear dots resembling nuclear bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • 8030451N04Rik antibody
      • AI413936 antibody
      • cb1085 antibody
      • DKFZp586K1123 antibody
      • E3 ubiquitin-protein ligase TRIM33 antibody
      • EC 6.3.2.- antibody
      • Ectodermin antibody
      • Ectodermin homolog antibody
      • FLJ11429 antibody
      • FLJ32925 antibody
      • id:ibd2175 antibody
      • MGC136680 antibody
      • mKIAA1113 antibody
      • OTTHUMP00000013662 antibody
      • OTTHUMP00000013663 antibody
      • Protein Rfg7 antibody
      • PTC7 antibody
      • Ret fused gene 7 antibody
      • RET-fused gene 7 protein antibody
      • RFG7 antibody
      • Rfg7 protein antibody
      • TF1G antibody
      • TIF1-gamma antibody
      • TIF1G antibody
      • TIF1GAMMA antibody
      • TIFGAMMA antibody
      • Transcription intermediary factor 1-gamma antibody
      • Transcriptional intermediary factor 1 gamma antibody
      • TRI33_HUMAN antibody
      • trim33 antibody
      • Tripartite motif containing 33 antibody
      • Tripartite motif containing 33 protein antibody
      • tripartite motif-containing 33 antibody
      • Tripartite motif-containing protein 33 antibody
      • wu:fc17f10 antibody
      • zgc:136680 antibody
      see all

    Anti-TIF1 gamma antibody [16G9] images

    References for Anti-TIF1 gamma antibody [16G9] (ab33475)

    ab33475 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply. I will forward your request to my colleague in our Hong Kong office, and she will add this vial to your next order. Please let me know if there is anything else that we can do for you.

    Thank you for contacting us. I am sorry to hear that ab33475 is producing high background in WB. After reviewing the customer's protocol and image, I agree that this antibody is not functioning properly. I would be happy to offer the custome...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (BxPC-3)
    Specification BxPC-3
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Dec 07 2006

    Thank you for your enquiry. For a positive control, please refer to the following article: Yan KP et al. Molecular cloning, genomic structure, and expression analysis of the mouse transcriptional intermediary factor 1 gamma gene. Gene 2004 Jun 9;334...

    Read More