Overview

  • Product nameAnti-TIF1 gamma antibody
    See all TIF1 gamma primary antibodies
  • Description
    Rabbit polyclonal to TIF1 gamma
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human TIF1 gamma.

    (Peptide available as ab47108.)

  • Positive control
    • ab47062 gave a positive signal in the following whole cell lysates: F9 (Mouse embryonic carcinoma cell line); E14tG2a (Mouse embryonic stem cell line); HeLa (Human epithelial carcinoma cell line) (data not shown); MEF1 (Mouse embryonic fibroblast cell line) (data not shown); MEL-1 (Human embryonic stem cell, male cell line) (data not shown); Mouse Embryonic Germ cell (data not shown).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 122 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionActs as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
  • Tissue specificityExpressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus. In discrete nuclear dots resembling nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8030451N04Rik antibody
    • AI413936 antibody
    • cb1085 antibody
    • DKFZp586K1123 antibody
    • E3 ubiquitin-protein ligase TRIM33 antibody
    • EC 6.3.2.- antibody
    • Ectodermin antibody
    • Ectodermin homolog antibody
    • FLJ11429 antibody
    • FLJ32925 antibody
    • id:ibd2175 antibody
    • MGC136680 antibody
    • mKIAA1113 antibody
    • OTTHUMP00000013662 antibody
    • OTTHUMP00000013663 antibody
    • Protein Rfg7 antibody
    • PTC7 antibody
    • Ret fused gene 7 antibody
    • RET-fused gene 7 protein antibody
    • RFG7 antibody
    • Rfg7 protein antibody
    • TF1G antibody
    • TIF1-gamma antibody
    • TIF1G antibody
    • TIF1GAMMA antibody
    • TIFGAMMA antibody
    • Transcription intermediary factor 1-gamma antibody
    • Transcriptional intermediary factor 1 gamma antibody
    • TRI33_HUMAN antibody
    • trim33 antibody
    • Tripartite motif containing 33 antibody
    • Tripartite motif containing 33 protein antibody
    • tripartite motif-containing 33 antibody
    • Tripartite motif-containing protein 33 antibody
    • wu:fc17f10 antibody
    • zgc:136680 antibody
    see all

Anti-TIF1 gamma antibody images

  • ICC/IF image of ab47062 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47062, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-TIF1 gamma antibody (ab47062) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
    Lane 2 : E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 220 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-TIF1 gamma antibody (ab47062) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 220 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-TIF1 gamma antibody (ab47062)

ab47062 has not yet been referenced specifically in any publications.

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