Overview

  • Product nameAnti-TIGAR antibody
    See all TIGAR primary antibodies
  • Description
    Rabbit polyclonal to TIGAR
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between amino acids 220 and 270 of Human TIGAR (NP_065108.1)

  • Positive control
    • HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab113980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
    • Sequence similaritiesBelongs to the phosphoglycerate mutase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6-bisphosphatase TIGAR antibody
      • C12ORF5 antibody
      • chromosome 12 open reading frame 5 antibody
      • FR2BP antibody
      • Fructose-2,6-bisphosphatase TIGAR antibody
      • Fructose-2,6-bisphosphate 2-phosphatase antibody
      • Probable fructose 2,6 bisphosphatase TIGAR antibody
      • Probable fructose-2 antibody
      • tigar antibody
      • TIGAR_HUMAN antibody
      • TP53 induced glycolysis and apoptosis regulator antibody
      • TP53 induced glycolysis regulatory phosphatase antibody
      • TP53-induced glycolysis and apoptosis regulator antibody
      • Transactivated by NS3TP2 protein antibody
      see all

    Anti-TIGAR antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TIGAR with ab113980 at 1/1000 (1µg/ml). Detection: DAB.
    • ab113980 used for IP at 10 µg/mg lysate, staining TIGAR in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded)

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-TIGAR antibody (ab113980)

    ab113980 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113980.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"